Biological effects of exosomes derived from 2D and 3D culture adipose stem cells on JEC Cell proliferation and migration.

ABSTRACT:

Objective

To explore the biological impacts of exosomes derived from adipose stem cells cultured under two-dimensional (2D) and three-dimensional (3D) conditions on endometrial adenocarcinoma cells (JEC cells).

Materials and methods

Exosomes were isolated and extracted from adipose stem cells grown in 2D and 3D cultures. JEC cells were allocated into three categories: a control group, a 2D group, and a 3D group. Cultivation of JEC cells occurred with distinct media (supplemented with or without 2D/3D adipose-derived stem cell exosomes) per group. Endometrial cancer cell viability was evaluated by a Cell Counting Kit-8 (CCK-8) assay. Scratch assays gauged the migratory behavior of endometrial adenocarcinoma cells. A Transwell assay quantified cellular invasiveness.

Results

SUBMITTER: Li Y 

PROVIDER: S-EPMC12287758 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature