Reducing dextran sodium sulfate interference with gene expression quantification in a mouse model of colitis.

ABSTRACT: Gene expression analysis via reverse transcription quantitative real-time polymerase chain reaction (qPCR) can be inhibited by various substances, including dextran sodium sulfate (DSS), a chemical commonly used to induce intestinal inflammation in animal models. Ensuring elimination and reduction of qPCR interference in tissues from laboratory animals following oral administration of DSS is critical and may improve the power of experimental tests. While methods for DSS removal have been reported, their effectiveness varies depending on the animal model, extraction techniques, DSS concentration, and treatment duration. We compared the effectiveness of the commonly used RNeasy Plus Universal Mini Kit with and without further lithium chloride (LiCl) precipitation at eliminating or reducing q

SUBMITTER: Hechter D 

PROVIDER: S-EPMC12288034 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature