Digital PCR outperforms quantitative real-time PCR for the detection and quantification of major periodontal pathobionts.

ABSTRACT:

Background

This study comparatively evaluated the analytical and diagnostic performance of a multiplex digital polymerase-chain reaction (dPCR) assay and a quantitative real-time PCR (qPCR) for the simultaneous detection and quantification of periodontal pathobionts: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum.

Materials and methods

Subgingival plaque samples from 20 periodontitis patients and 20 periodontally healthy controls were analyzed. Several analytical parameters of the dPCR assay, optimized using DNA standards, were assessed versus qPCR: dynamic range linearity, precision, accuracy, prevalence, sensitivity, specificity, and concordance. The statistical analyses included Mann-Whitney U test, Wilcox

SUBMITTER: Munjakovic H 

PROVIDER: S-EPMC12288185 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature