Proteoform-predictor: Increasing the Phylogenetic Reach of Top-Down Proteomics.

ABSTRACT: Proteoforms are distinct molecular forms of proteins that act as building blocks of organisms, with post-translational modifications (PTMs) being one of the key changes that generate these variations. Mass spectrometry (MS)-based top-down proteomics (TDP) is the leading technology for proteoform identification due to its preservation of intact proteoforms for analysis, making it well-suited for comprehensive PTM characterization. A crucial step in TDP is searching MS data against a database of candidate proteoforms. To extend the reach of TDP to organisms with limited PTM annotations, we developed Proteoform-predictor, an open-source tool that integrates homology-based PTM site prediction into proteoform database creation. The new tool creates databases of proteoform candidates after regis

SUBMITTER: Su T 

PROVIDER: S-EPMC12288289 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature