Identification of electron transfer enzymes in <i>Thermoanaerobacterium saccharolyticum</i>.

ABSTRACT: Thermoanaerobacterium saccharolyticum is a promising candidate for the production of biofuels from lignocellulosic sugars; however, the genes associated with electron transfer from ferredoxin are poorly characterized. In this work, we deleted several key electron transfer genes. We showed that the tsac_1705 gene is not necessary for high-yield ethanol production, but that a set of four other genes (nfnA, nfnB, hfsD, and hydA) are necessary. We showed that the nfnB gene can function as a monofunctional (i.e., non-bifurcating) FNOR enzyme in the absence of nfnA. The phenotypes of the hfsD, hydA, and hfsD hydA double-deletion strains are consistent with their function via hydrogen cycling.

Importance

The impro

SUBMITTER: Fabri JHTM 

PROVIDER: S-EPMC12288451 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature