Molecular characterization and resistance mechanisms of ertapenem-non-susceptible carbapenem-resistant <i>Klebsiella pneumoniae</i> co-harboring ESBLs or AmpC enzymes with porin loss or efflux pump overexpression.

ABSTRACT: Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a significant challenge in clinical settings due to limited treatment options and high mortality rates. While carbapenemase-producing CRKP has been extensively studied, the mechanisms underlying resistance in non-carbapenemase-producing CRKP remain less understood. In this study, we investigated 30 ertapenem-resistant non-carbapenemase-producing CRKP clinical isolates. Antimicrobial susceptibility testing revealed multidrug resistance in all strains. Molecular analyses showed that all isolates carried extended-spectrum β-lactamase (ESBL) and/or AmpC β-lactamase genes. Sequencing of outer membrane porin (OMP) genes revealed mutations or deletions in ompK35 and/or ompK36 in the majority of isolates. SDS-PAGE analysi

SUBMITTER: Su Y 

PROVIDER: S-EPMC12288452 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature