Conjugative delivery of toxin genes <i>ccdB</i> and <i>kil</i> confers synergistic killing of bacterial recipients.

ABSTRACT: The bacterial type IV secretion systems (T4SS) are medically problematic for their roles in the dissemination of mobile genetic elements or effector proteins, but they also have great potential for new antimicrobial therapies. Recent studies have deployed the T4SS subfamily of conjugation systems to deliver gene editing CRISPR/Cas systems to disrupt drug resistance genes or kill targeted bacterial recipients. However, the therapeutic potential of conjugative CRISPR/Cas delivery is compromised by mutations or host repair systems that diminish the efficiency with which CRISPR/Cas induces double-strand breaks in new transconjugants. Here, we compared the efficiencies of conjugation-based killing systems based on the delivery of CRISPR-Cas9 elements or toxin genes encoding the bacteriophage la

SUBMITTER: Li YG 

PROVIDER: S-EPMC12288468 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature