DdiA, an XRE family transcriptional regulator, is a co-regulator of the DNA damage response in Myxococcus xanthus.

ABSTRACT: Repair of DNA damage is essential for genome integrity. DNA damage elicits a DNA damage response (DDR) that includes error-free and error-prone, i.e., mutagenic, repair. The SOS response is a widely conserved system in bacteria that regulates the DDR and depends on the recombinase RecA and the transcriptional repressor LexA. However, RecA/LexA-independent DDRs have been identified in several bacterial species. Here, using a whole-cell, label-free quantitative proteomics approach, we map the proteomic response in Myxococcus xanthus to mitomycin C treatment and the lack of LexA. In doing so, we demonstrate a LexA-independent proteomic DDR in M. xanthus. Using a candidate approach, we identify DNA damage-induced protein A (DdiA), a transcriptional reg

SUBMITTER: Jung J 

PROVIDER: S-EPMC12288472 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature