Atomic Force Microscopy Reveals Differences In Mechanical Properties Linked To Cortical Structure In Mouse And Human Oocytes.

ABSTRACT: Cell mechanical properties regulate biological processes such as oocyte development. Cortical tension is regulated via actomyosin cortex remodeling to ensure optimal oocyte quality. However, the evolution of other mechanical parameters and their relationship with cortex structure remain poorly understood in mammalian oocytes. In this work, a methodology combining multiple mechanical parameters measured through Atomic Force Microscopy is proposed to investigate the relationship between oocyte mechanical properties and cortex organization. By studying mouse oocytes at various stages of development, along with engineered ones with specific cortex organization, it is demonstrated that a thin actin cortex corresponds to stiff oocytes while a thick one is associated with softer oocytes. It is fu

SUBMITTER: Bulteau R 

PROVIDER: S-EPMC12288798 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature