Device-free isolation of photoreceptor cells from patient iPSC-derived retinal organoids.

ABSTRACT: Autologous photoreceptor cell replacement is one of the most promising strategies currently being developed for the treatment of patients with inherited retinal degenerative blindness. Induced pluripotent stem cell-derived (iPSC-derived) retinal organoids, which faithfully recapitulate the structure of the neural retina, are an ideal source of transplantable photoreceptors required for these therapies. However, retinal organoids contain other retinal cell types, including bipolar, horizontal, and amacrine cells, which are unneeded and may reduce the potency of the final therapeutic product. Therefore, approaches for isolating fate-committed photoreceptor cells from dissociated retinal organoids are desirable. In this work, we present partial dissociation, a technique that leverages the hig

SUBMITTER: Stone NE 

PROVIDER: S-EPMC12288960 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature