Anti-Mullerian hormone binds heat shock protein glucose-regulated protein 78 in human follicular fluid.

ABSTRACT:

Purpose

Anti-Müllerian hormone (AMH) regulates key steps in folliculogenesis, yet unlike other TGF-β family members, no soluble AMH-binding proteins have been identified. We hypothesized that AMH may bind shuttling proteins in human follicular fluid (FF).

Methods

Discarded pooled FF was collected from women undergoing oocyte retrieval at an academic fertility center. AMH-binding soluble proteins in FF were co-immunoprecipitated with AMH, identified by mass spectrometry, and their association was validated by western blot. We characterized the spatiotemporal expression of candidate binding protein glucose-regulated protein 78 (GRP78) in the mouse ovary across the cycle by immunohistochemistry. We used enzyme-linked immunoassay (ELISA) to measure AMH and GRP78 concentrations i

SUBMITTER: Sacha CR 

PROVIDER: S-EPMC12289096 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature