Aloin protects against UVB-induced apoptosis by modulating integrated signaling pathways.

ABSTRACT: Aloin, an anthraquinone compound, is naturally abundant in the Aloe. This study comprehensively investigates the photoprotective effects of aloin against UVB-induced damage in HaCaT cells, elucidating its antioxidant capacity and its role in preventing cellular apoptosis. Aloin demonstrated significant antioxidant activity in ABTS and DPPH assays, with a dose-dependent reduction in intracellular reactive oxygen species levels as evidenced by fluorescence analysis. Western blot analysis revealed that aloin inhibited the phosphorylation of both p38 and JNK, with a more pronounced effect on p38. This was further supported by IC50 values, indicating a higher inhibitory potency of aloin against p38 compared to JNK. Assessments using MTT, Hoechst, Calcein/PI staining, and flow cytometry c

SUBMITTER: He Q 

PROVIDER: S-EPMC12289626 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature