Preparation, characterization and antioxidative activity analysis of recombinant humanized collagen type III-loaded liposomes.

ABSTRACT: To improve the bioavailability and biological activity of recombinant type III humanized collagen (rhCol III), we optimized the preparation process of recombinant type III humanized collagen-loaded liposomes (rhCol III-LIPS) using the film dispersion and periplasmic extrusion methods, with the encapsulation rate as the response value. Then we analyzed the antioxidative activity of rhCol III-LIPS by determining the scavenging rate of free radicals. We investigated the protective effect of rhCol III-LIPS against H₂O₂-induced injuries in HaCaT cells by detecting cell vitality, contents of reactive oxygen species (ROS), malondialdehyde (MDA), and activities of antioxidases superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase dismutase (GSH-Px). The results s

SUBMITTER: Du M 

PROVIDER: S-EPMC12289889 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature