Extracellular vesicles as biomarkers for traumatic brain injury using a 3D in vitro human brain tissue model.

ABSTRACT: Traumatic brain injury (TBI) is a significant health challenge worldwide, with current diagnostics and treatment falling short due to the complex pathophysiology involved. Extracellular vesicles (EVs) play a crucial role in brain injury response and are promising biomarkers for understanding the progression of TBI. A 3D in vitro human brain tissue model, comprising neurons, astrocytes, and microglia was utilized to simulate TBI and investigate EV responses. EVs were isolated at multiple acute timepoints post-injury and microRNA (miRNA) profiling revealed transient dysregulation of several miRNAs that aligned with clinical and in vivo studies. Pathway analysis revealed that these miRNAs are associated with the phosphoinositide 3-kinase / protein kinase B (PI3K / AKT) cell signaling pathway,

SUBMITTER: Hsi P 

PROVIDER: S-EPMC12289892 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature