Construction of dynamic ceRNA regulatory networks in osteogenesis during fracture healing based on transcriptomic analysis.

ABSTRACT: Fracture healing is a complex biological process. This study aimed to investigate the key molecules involved in fracture healing and their potential competing endogenous RNA (ceRNA) regulatory mechanisms within the first 28 days post-fracture using bioinformatics methods. The experiment was conducted on 15 adult male SD rats, with tibia callus tissue samples collected at days 0, 3, 7, 14, and 28 (n = 3) post-fracture. RNA-Seq was used for high-throughput transcriptome sequencing, followed by differential expression analysis to identify differentially expressed genes (DEGs), long non-coding RNA (DELs), and microRNA (DEMs) at different stages. Protein-protein interaction (PPI) networks were constructed using the STRING database and visualized with Cytoscape. GO and KEGG enrichment analyses w

SUBMITTER: Guo S 

PROVIDER: S-EPMC12289964 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature