Modeling proximalisation in axolotl limb regeneration.

ABSTRACT: The axolotl (Ambystoma mexicanum) possesses a remarkable ability to regenerate tissues. Following limb amputation, a blastema of progenitor cells forms, expands, and reconstructs all distal structures, implying that mature cells near the wound retain positional memory along the proximal-distal (PD) axis. Key regulators of positional identity, such as Prod1 and Tig1, promote proximalisation-a shift toward a more proximal identity-when overexpressed, but the mechanisms governing this process remain unclear. In this study, we tracked changes in cellular density along the PD axis of regenerating axolotl limbs after transfecting distal blastemas with Tig1 and Prod1, mapping the spatiotemporal distribution of transfected cells and their progeny throughout regeneration. Using a continuous mathema

SUBMITTER: Arce H 

PROVIDER: S-EPMC12290015 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature