Investigation of molecular mechanisms in silver nanoparticle-induced cytotoxicity from gene to metabolite level.

ABSTRACT: The aim of this study was to explore the mechanisms in silver nanoparticle (AgNP)-induced cytotoxicity from gene to metabolite levels through an integrative analysis of transcriptomics and metabolomics results. First, transcriptome sequencing technology revealed 1365, 1241, and 2790 genes differentially expressed within human dermal fibroblasts (HDFs) after 4, 8, and 24 h of exposure to silver nanoparticles, which were involved in 250, 248, and 280 biological pathways. Then, by comparing with the metabolomics results, 7 metabolic pathways (purine metabolism pathway, glycerophospholipid metabolism pathway, etc.), with 9 key upstream genes (ADCY4, SPHK1, etc.) and 8 downstream metabolites (xanthine, choline, etc.) jointly involved were found relate to AgNP-induced cytotoxicity. Finally, the

SUBMITTER: Huang Y 

PROVIDER: S-EPMC12290054 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature