In-depth amino acid mutational analysis of the key interspecific incompatibility factor Stigmatic Privacy 1.

ABSTRACT: In plants, there is an active prezygotic interspecific-incompatibility mechanism to prevent unfavorable hybrids between two species. We previously reported that an uncharacterized protein with four-transmembrane domains, named as Stigmatic Privacy 1 (SPRI1), is responsible for rejecting heterospecific pollen grains in Arabidopsis thaliana. However, the lack of notable functional domains in SPRI1 has limited our understanding of its biochemical properties. In this study, we conducted a functional analysis of the SPRI1 protein through point-mutational experiments and biochemical analysis. We explored the molecular regulatory mechanisms of SPRI1 and the relationships with its function. Alanine- and glycine-scanning experiments together with the evolutional analysis showed that the structural

SUBMITTER: Kato Y 

PROVIDER: S-EPMC12290284 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature