Comprehensive high-throughput sequencing analysis and verification of miRNAs isolated from bronchial epithelial cells undergoing radiation-induced epithelial-mesenchymal transition.

ABSTRACT: Radiation-induced pulmonary fibrosis (RIPF) constitutes a significant complication in radiotherapy for various cancers, often severely limiting its efficacy. Recent studies suggest that epithelial-mesenchymal transition (EMT) plays a vital role in the pathogenesis of RIPF. Elucidating the involvement of microRNAs (miRNAs) in EMT could provide valuable insights into the mechanisms underlying RIPF and potentially reveal therapeutic targets. In this study, twelve dishes of BEAS-2B cells were irradiated with 6 Gy ⁶⁰Co γ-rays, and RNA was extracted at 0, 6, and 48 h after irradiation. High-throughput sequencing analysis of miRNA samples revealed miRNA significant changes in the BEAS-2B cells after irradiation, which was verified by RT-PCR. Additionally, the upstream transcription fact

SUBMITTER: Hou Y 

PROVIDER: S-EPMC12290335 | biostudies-literature | 2025 Jul-Sep

REPOSITORIES: biostudies-literature