Chromatic aberration (Cc) corrected cryo-EM: The structure of pseudorabies virus (PRV) using both zero-loss and energy loss electrons.

ABSTRACT: Here we have investigated the potential improvement in imaging vitrified biological specimens with the help of a chromatic aberration (Cc)-corrector. Using a newly developed chromatic aberration-corrected electron cryomicroscope (cryo-EM), the phase contrast micrographs comprising signals from both the zero loss and low energy loss (1-100 eV) channels were used to determine the structure of a pseudorabies virus (PRV). Using an energy selecting, electron energy loss spectrometer after the Cc corrector, datasets were collected separately yet sequentially on the same specimen to allow quantification of the signal in each of the respective channels. Both zero-loss first and low-loss first datasets were acquired. For further comparison, datasets from non-Cc-corrected cryo-EM were also collected

SUBMITTER: Wu J 

PROVIDER: S-EPMC12290466 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature