Microfluidic Paper-Based Electrochemical Immunosensor for the Detection of Prostate-Specific Antigen (PSA) Based on Modified Flexible Screen-Printed Carbon Electrodes.

ABSTRACT: A microfluidic paper-based electrochemical immunosensor (μPAD) presents a promising platform for detecting prostate-specific antigen (PSA), a key biomarker for prostate cancer. Detection is based on the unique antigen-antibody binding, which restricts electron transfer at the electrode's surface. In this study, we developed a label-free immunosensor using ferri-/ferrocyanide ([Fe-(CN)₆]^3-/^4-) as a redox probe to monitor current changes before and after antigen immobilization. The screen-printed carbon electrode (SPCE) was modified with mesoporous carbon (MC) to boost the electrochemical signals due to its high surface area and functional carboxylic acid groups for efficient antibody attachment. By integrating this with a μPAD, sample handling and preparatio

SUBMITTER: Soongsong J 

PROVIDER: S-EPMC12290616 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature