Analytical and Structural Evaluation of Recombinant Human Serum Albumin and Fragment F8 for Aptamer-Based Urinary Biomarker Detection.

ABSTRACT: Human serum albumin (HSA) is a key biomarker for early detection of kidney disorders such as microalbuminuria. However, its structural complexity and susceptibility to degradation in urine challenge current diagnostic assays, particularly immunoturbidimetric methods that depend on epitope stability. In this study, we expressed and characterized full-length recombinant HSA (rHSA) and its proteolytic fragment F8 (residues 496-585) in both and rice-based systems to explore their suitability for biosensor validation and diagnostic use. Comparative analysis via SDS-PAGE, LC-MS/MS, and dynamic light scattering revealed differences in solubility and aggregation profiles between expression systems, with rice-derived rHSA showing greater resistance to proteolytic degradation. The F8 fragment retain

SUBMITTER: Archapraditkul C 

PROVIDER: S-EPMC12290929 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature