Solvent Exclusion Effect on Infrared Absorption Spectroscopy.

ABSTRACT: Absorption spectroscopy of a solution containing an analyte solute typically uses the transmission through a solvent as a reference based on the assumption that the solvent does not absorb light. However, when a solvent absorbs light, the resulting absorbance becomes lower and often negative because of the reduced number of solvent molecules due to the space taken by solute molecules. The solvent exclusion (SE) effect is problematic for accurate quantitation and analysis across various absorption spectroscopies, especially near the broad, strong water absorption peaks. The commonly practiced method of subtracting a scaled reference water spectrum seems ideal; however, this requires extensively broad spectra that are not available with the combination of flow cell chamber thickness and curr

SUBMITTER: Lee YJ 

PROVIDER: S-EPMC12291039 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature