Detection and Analysis of Reactive Oxygen Species (ROS): Buffer Components Are Not Bystanders.

ABSTRACT: Reactive oxygen species (ROS) play critical roles in pathophysiological processes. Therefore, there is widespread interest in learning ROS concentrations under various conditions. However, literature numbers in ROS concentration vary significantly, and most cannot be readily compared against each other, largely because of the lack of understanding of the effects of various factors that significantly impact the experimental outcome. In this study, we examine an overlooked factor: the chemical reactivity of commonly used organic buffer molecules toward ROS and how such reactivity affects the results interpretation. Specifically, we examined HEPES, Tris, MES, citrate, ammonium acetate, and phosphate-buffered saline (PBS) and found that most organic buffer components can rapidly consume NaOCl

SUBMITTER: Bansal S 

PROVIDER: S-EPMC12291042 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature