Peroxynitrite-Activated Conditional Chemical Probe for the Affinity-Based Protein Modification.

ABSTRACT: Affinity-based protein labeling chemical probes are invaluable tools for the selective chemical modification of native proteins. This protein labeling strategy generally relies on "always ON" reactive electrophiles to achieve protein modification. However, reactive electrophiles are also the causes of probe instability and nonselective labeling in complex biological environments. In this paper, we introduce a conditional-activated affinity-based protein labeling strategy in which the probe is activated by peroxynitrite, triggering a labeling reaction with the target protein located in the near proximity. This conditionally activated protein labeling probe was synthesized with a peroxynitrite-responsive acyl hydrazide, a Cy5 fluorescent dye, and a sulfonamide ligand for recognition with hum

SUBMITTER: Wu CC 

PROVIDER: S-EPMC12291043 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature