Fast and Accurate Charge State Deconvolution of Protein Mass Spectra.

ABSTRACT: Charge state deconvolution is essential for efficient and effective protein mass spectrometry analysis. High-quality mass profiling is necessary to determine which proteoforms are present in protein samples and their relative abundances. In the pursuit of a well-rounded deconvolution solution, we detail an iterative charge state deconvolution algorithm named kDecon that has been tuned to provide high accuracy in its mass results while also delivering superb sensitivity toward lower abundance proteoforms in complex spectra. Here, the performance of kDecon as a mass determination algorithm for both targeted antibody and high-throughput proteomics analysis was benchmarked against existing deconvolution solutions. While the different deconvolution routines all proved robust for detecting the h

SUBMITTER: Durbin KR 

PROVIDER: S-EPMC12291047 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature