Silencing P2Y₁₂ and P2Y₁₃ receptors rehabilitates the ADP-induced P2Y₁-mediated osteogenic commitment of post-menopausal mesenchymal stromal cells.

ABSTRACT:

Background

Participation of ADP-sensitive metabotropic P2Y₁, P2Y₁₂ and P2Y₁₃ receptors in human osteogenesis is controversial. Here, we investigated the variations in the expression and bone-forming properties of the P2Y₁R in osteogenic-differentiating bone marrow-derived mesenchymal stromal cells (BM-MSCs) isolated from post-menopausal (Pm) women. We also tested whether observed P2Y₁-related functional deficits result from the crosstalk with co-localized P2Y₁₂ and P2Y₁₃ receptors.

Methods

Pm BM-MSCs were cultured in an osteogenic-inducing medium in either the absence or presence of the selective P2Y₁ receptor agonist, MR2365; this compound was applied alone or after cells' incubation wit

SUBMITTER: Bessa-Andres C 

PROVIDER: S-EPMC12291242 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature