Multistep 11-<i>cis</i> to All-<i>trans</i> Retinal Photoisomerization in Bestrhodopsin, an Unusual Microbial Rhodopsin.

ABSTRACT: Rhodopsins constitute a broad class of retinal-binding photoreceptors. Microbial rhodopsins are canonically activated through an all-trans to 13-cis photoisomerization, whereas animal rhodopsins are mostly activated through an 11-cis to all-trans isomerization. Bestrhodopsins constitute a special microbial rhodopsin subfamily, with bistable rhodopsin domains that can be photoswitched between a far red-absorbing state D661 and a green-absorbing state P540. Its photochemistry involves a peculiar all-trans to 11-cis isomerization for the D661 to P540 photoreaction and vice versa. Here, we present the P. antarctica bestrhodopsin 11-cis to all-trans photoreaction as determined by femtosecond-to-submillisecond transient absorp

SUBMITTER: Broser M 

PROVIDER: S-EPMC12291454 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature