Multivalency Controls the Growth and Dynamics of a Biomolecular Condensate.

ABSTRACT: Biomolecular condensates are essential for cellular organization and function, yet understanding how chemical and physical factors govern their formation and dynamics has been limited by a lack of noninvasive measurement techniques. Conventional microscopy methods often rely on fluorescent labeling and substrate immobilization, which can perturb the intrinsic properties of condensates. To overcome these challenges, we apply label-free, contact-free holographic video microscopy to study the behavior of a condensate-forming protein in vitro. This technique enables rapid, high-throughput, and precise measurements of individual condensate diameters and refractive indexes, providing unprecedented insight into size distributions and dense-phase macromolecular concentrations over time. Using this

SUBMITTER: von Hofe J 

PROVIDER: S-EPMC12291466 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature