Immunomodulatory Effects of <i>Escherichia coli</i> Phage GADS24 on Human Dendritic Cells.

ABSTRACT: Background: Multidrug-resistant (MDR) Escherichia coli (E. coli) strains pose a significant public health challenge, which has led to the exploration of alternative therapeutic strategies. Due to their antibacterial and immunomodulatory properties, bacteriophages have emerged as promising therapeutic agents. Methods: This study investigates the effects of GADS24, a novel lytic bacteriophage of E. coli, on human-monocyte-derived dendritic cells (DCs). DCs are exposed to purified GADS24 phage, bacterial lysate, or a combination of both. Flow cytometry was used to assess the expression of surface markers (HLA-DR, CD80, CD83, and CD86), and ELISA was used to measure cytokine production (IL-10 and IL-12p70). Results: Following treatment with bacterial l

SUBMITTER: Aldahlawi AM 

PROVIDER: S-EPMC12292600 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature