ENaC Biomarker Detection in Platelets Using a Lateral Flow Immunoassay: A Clinical Validation Study.

ABSTRACT: Arterial hypertension (HTN) is a growing global health concern, with limited tools available for early detection. Previous studies identified the overexpression of the epithelial sodium channel (ENaC) as a potential biomarker for HTN. In this work, we optimized and clinically validated a lateral flow immunoassay (LFIA) using gold nanoparticles (AuNPs) functionalized with anti-ENaC antibodies. The test strips were prepared with 10 µL of each component and performed in a 9-point herringbone format. For validation, a double-blind study was conducted using platelet lysates from 200 individuals, classified based on real-time blood pressure measurements. ENaC expression was assessed via both LFIA and Western blotting, which served as the reference method. Receiver operating characteristic (ROC)

SUBMITTER: Martinez-Bolona G 

PROVIDER: S-EPMC12292876 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature