Pulsed dipolar hyperfine spectroscopy for molecular distance measurements in the angstrom to nanometer scale.

ABSTRACT: Hyperfine spectroscopy is a fundamental method in biophysical and material sciences to detect nuclear spins in vicinity of paramagnetic centers, leading to molecular structural information. Among variants of this experiment, only electron-nuclear double resonance (ENDOR) has been established to detect nuclei at interspin distances up to about 1.7 nanometers using ¹⁹F labels. This limit is dictated by the ENDOR linewidth of 10 to 30 kilohertz, which appeared insurmountable given dipolar broadening of the detected nucleus with the nuclear spin bath. Herein, we present ENDOR experiments based on nuclear sublevel coherence spectroscopy that push the boundaries of ENDOR sensitivity and resolution by one order of magnitude. In particular, we introduce an experiment, in which the elect

SUBMITTER: Sielaff L 

PROVIDER: S-EPMC12292905 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature