A High-Throughput ImmunoHistoFluorescence (IHF) Method for Sub-Nuclear Protein Analysis in Tissue.

ABSTRACT: The current understanding of cellular protein distribution in clinical samples is limited. This is partially due to the complexity and heterogeneity of tissues combined with the qualitative nature of analysis by immunohistochemistry (IHC). The common use of manual assessment in the clinic is time-consuming and restricts both the complexity of scoring and the scale of patient tissue analysis. This has limited the transfer of biological observations into pathology and their integration into diagnostics. Immunofluorescence (IF) techniques allow detailed and high-throughput investigation of proteins in cell models, but their application to tissues has been hindered by poor antibody penetration, autofluorescence artefacts, and weak signals. With a growing focus on precision medicine, scalable t

SUBMITTER: Oxe KC 

PROVIDER: S-EPMC12293571 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature