An Automated Microfluidic Platform for In Vitro Raman Analysis of Living Cells.

ABSTRACT: We present a miniaturized, inexpensive, and user-friendly microfluidic platform to support biological applications. The system integrates a mini-incubator providing controlled environmental conditions and housing a microfluidic device for long-term cell culture experiments. The incubator is designed to be compatible with standard inverted optical microscopes and Raman spectrometers, allowing for the non-invasive imaging and spectroscopic analysis of cell cultures in vitro. The microfluidic device, which reproduces a dynamic environment, was optimized to sustain a passive, gravity-driven flow of medium, eliminating the need for an external pumping system and reducing mechanical stress on the cells. The platform was tested using Raman analysis and adherent tumoral cells to assess proliferati

SUBMITTER: Klyusko I 

PROVIDER: S-EPMC12293637 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature