Mechanistic Insights into the Bornyl Diphosphate Synthase from <i>Lavandula angustifolia</i>.

ABSTRACT: Lavender species hold substantial economic importance due to their widespread cultivation for essential oils (EOs). Lavender EOs contain terpenes essential for industries such as cosmetics, personal care, and pharmaceuticals. In the biosynthetic pathway of EOs, Lavandula angustifolia bornyl diphosphate synthase (LaBPPS) catalyzes the conversion of geranyl diphosphate (GPP) to bornyl diphosphate (BPP). However, the functional mechanisms of LaBPPS remain poorly understood. Here, we conducted mutational experiments based on the molecular docking results, and found that mutations at positions D356A, D360A, R497A, D501A, or E508A led to a 50- to 100-fold reduction in the activity. Deletion of region 1-58 (∆1-58) did not affect activity compared to the wild-type (WT) protein, while deleti

SUBMITTER: Liu D 

PROVIDER: S-EPMC12293770 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature