Flexible Hydrophobic Paper-Based Microfluidic Field-Effect Biosensor Amplified by RNA-Cleaving DNAzyme-Based DNA Nanostructure for Mg²⁺ Detection.

ABSTRACT: Magnesium ions (Mg²⁺) play an important role in animal health, with their concentration in the bloodstream serving as a key indicator for hypomagnesemia diagnosis. In this study, a flexible hydrophobic paper-based microfluidic field-effect biosensor was developed for point-of-care Mg²⁺ detection, which integrated flexible hydrophobic paper, semiconducting single-walled carbon nanotubes (SWNTs) and a Mg²⁺-specific RNA-cleaving DNAzyme(RCD)-based DNA nanostructure. Flexible hydrophobic paper was synthesized by using cellulose paper and octadecyltrichlorosilane, improving mechanical strength and decreasing biological interference. To achieve high sensitivity, the Mg²⁺-specific RCD was functionalized with SWNTs, and then repeatedly self-assembled two

SUBMITTER: Wang H 

PROVIDER: S-EPMC12293950 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature