Droplet-Based Measurements of DNA-Templated Nanoclusters-Towards Point-of-Care Applications.

ABSTRACT: In this work, we investigate the fundamental usability of fluorescent DNA-templated silver nanoclusters (DNA-AgNCs) as sensors for Point-of Care-Testing (PoCT) applications. We developed a microfluidic platform for the generation of droplets containing DNA-AgNCs in defined, different chemical environments. The droplets are read out fluorescently at two different emission wavelengths. For the pre-evaluation for the usage of biologically relevant matrices with DNA-AgNCs, the response of two different DNA-AgNCs to a variation in pH and sodium chloride concentration was acquired. Our compact and simple setup can detect DNA-AgNCs well below 100 nM and allows the characterization of the fluorescence response of DNA-based biohybrid nanosensors to changes in the chemical environment within short m

SUBMITTER: Kluitmann J 

PROVIDER: S-EPMC12294068 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature