Biocompatibility of Hydraulic Calcium Silicate-Based Cement MTA Flow^TM on Human Dental Pulp Stem Cells In Vitro.

ABSTRACT:

Aim

hydraulic calcium silicate-based cements (HCSCs) are widely used in endodontics for vital pulp therapy and other clinical procedures due to their favorable physicochemical and biological properties. This study evaluates the biological properties of two HCSCs-MTA Flow™ and MTA Flow™ White (in a 3:2 liquid-to-powder ratio, thick consistency)-on human dental pulp stem cells (hDPSCs).

Methodology

hDPSCs were exposed to leachates from MTA Flow™, MTA Flow™ White, and ProRoot^® MTA. pH changes, cytotoxicity, cell proliferation, cell morphology, and cell death (apoptosis/necrosis) were assessed in vitro.

Results

MTA Flow™ White and ProRoot^® MTA leachates produced a strongly alkaline pH (~10-12) compared to the negative control, whereas MTA Flow™ leach

SUBMITTER: Tusas P 

PROVIDER: S-EPMC12294829 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature