Droplet Digital PCR Improves Detection of <i>BRCA1/2</i> Copy Number Variants in Advanced Prostate Cancer.

ABSTRACT: BRCA1 and BRCA2 are associated with advanced prostate cancer progression and poor prognosis. Copy number variants (CNVs) of these genes play a crucial role in guiding targeted treatments, particularly for patients receiving PARP inhibitors. However, CNV detection using multiplex ligation-dependent probe amplification (MLPA) is often limited by tumor heterogeneity, leading to ambiguous results. This study therefore aimed to evaluate BRCA1/2 CNVs in advanced prostate cancer patients using droplet digital PCR (ddPCR) and compare the results with MLPA. DNA from 11 advanced prostate cancer tissues was analyzed using both methods, in parallel with four cell lines and seven healthy volunteers. Our findings revealed that ddPCR effectively classified normal CNV groups-including

SUBMITTER: Rungkamoltip P 

PROVIDER: S-EPMC12295428 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature