Identifying promoters to enhance heterologous gene expression in recombinant Saccharomyces cerevisiae strains cultivated on non-native substrates.

ABSTRACT: Efficient bioconversion of lignocellulosic biomass (LCB) to ethanol by Saccharomyces cerevisiae requires its engineering to express heterologous enzymes at titres high enough to make significant impacts on industrial consolidated bioprocessing (CBP). Promoters are required for this purpose, but are reportedly influenced by various environmental factors as well as the protein specific nature of expression, warranting the need for assessment under the conditions for which they are intended. Heterologous xylosidase- and xylanase-encoding genes (xln43_SED1 and xyn2) were individually cloned under transcriptional control of the SED1_P and TDH3_P promoters, and DIT1_T terminator, and integrated into the genome of an a S. cerevisiae strain engineered for xylose utili

SUBMITTER: Fortuin J 

PROVIDER: S-EPMC12296805 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature