A systematic analysis of the network of lncRNAs and mRNAs regulated by TP53 and TP53 mutants with hotspot mutations.

ABSTRACT: The transcription factor TP53 exhibits the preeminent frequency of genetic mutations across various cancer types. Long non-coding RNAs (lncRNAs) stand as pivotal molecules in the initiation and progression of carcinogenesis. Nonetheless, the specific roles of TP53-regulated lncRNAs in colon cancer remain largely unexplored. In this study, we conducted a comprehensive analysis of lncRNA and mRNA alterations in DLD1 colon cancer cells, induced by the overexpression of wild-type TP53, as well as two TP53 hotspot mutations, namely TP53-R175H and TP53-R175P, leveraging transcriptomic deep sequencing technology. Across all three experimental groups, large-scale datasets encompassing approximately 300 lncRNAs and 1000 mRNAs were identified. Integrative analyses, employing KEGG and Reactome functi

SUBMITTER: Chen H 

PROVIDER: S-EPMC12297704 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature