Electroporation- and Liposome-Mediated Co-Transfection of Single and Multiple Plasmids.

ABSTRACT: Background/Objectives: Co-transfection of multiple DNAs is important to many research and therapeutic applications. While the optimization of single plasmid transfection is common, multiple plasmid co-transfection analyses are limited. Here we provide empirical data regarding multiple plasmid co-transfection while altering the number of species of plasmids transfected (up to four different plasmids) and the amount of plasmids/cell using the two most common non-viral techniques, electroporation and lipofection. Methods: A549 human lung epithelial cells were transfected using lipofectamine 2000 or electroporation with combinations of plasmids, each expressing one of four different fluorescent proteins from the CAGG promoter. Twenty-four hours later, cells were analyzed by spect

SUBMITTER: Baliga UK 

PROVIDER: S-EPMC12297979 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature