DNA from <i>Lactobacillus paragasseri</i> SBT2055 Activates Plasmacytoid Dendritic Cells and Induces IFN-α via TLR9.

ABSTRACT: Previously, we reported that Lactobacillus paragasseri SBT2055 (LG2055) activates plasmacytoid dendritic cells (pDCs) and induces interferon alpha (IFN-α) in vitro. Our clinical trial suggested that LG2055 intake may enhance pDC activity, supporting immune maintenance and reducing subjective common cold symptoms. However, the precise mechanisms remain unclear. In this study, we investigated how LG2055 engages with pDCs to stimulate IFN-α production. We evaluated LG2055-induced pDC activation using flow cytometry, ELISA, and phagocytosis assays. Human peripheral blood mononuclear cells (PBMCs) were stimulated with LG2055 and its components to evaluate immune responses. An in vitro M cell model was used to examine LG2055 translocation. We found that DNA extracted from LG2055 activated

SUBMITTER: Kobatake E 

PROVIDER: S-EPMC12298028 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature