Rapamycin Plays a Pivotal Role in the Potent Antifungal Activity Exhibited Against <i>Verticillium dahliae</i> by <i>Streptomyces iranensis</i> OE54 and <i>Streptomyces lacaronensis</i> sp. nov. Isolated from Olive Roots.

ABSTRACT: Verticillium wilt, caused by Verticillium dahliae, poses a significant threat to olive trees (Olea europaea L.). The isolation of endophytic Streptomyces strains from olive roots has led to the discovery of several strains showing strong antifungal activity against V. dahliae, as demonstrated through in vitro and small-scale soil experiments. Molecular analyses confirmed that strain OE54 belongs to Streptomyces iranensis. The main antifungal compound identified in this strain was rapamycin. Rapamycin displayed potent antifungal effects, notably inhibiting conidiospore germination (IC₅₀ = 87.36 μg/mL) and the hyphal growth of V. dahliae, with a minimum inhibitory concentration (MIC₅₀) of 3.91 ng/mL. Additionally, a second rapam

SUBMITTER: Calvo-Pena C 

PROVIDER: S-EPMC12298158 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature