Disruptive DNA Intercalation Is the Mode of Interaction Behind Niacinamide Antimicrobial Activity.

ABSTRACT: Niacinamide was recently shown to directly interact with bacterial DNA and interfere with cell replication; niacinamide mode of interaction and efficacy as a natural anti-microbial molecule were also described. The aim of this study is to elucidate the exact binding mechanism of niacinamide to microbial DNA. Intercalation is a binding mode where a small planar molecule, such as niacinamide, is inserted between base pairs, causing structural changes in the DNA. Melting curve analysis with various intercalating dyes demonstrated that niacinamide interaction with bacterial DNA reduces its melting temperature in a linear dose-dependent manner. Niacinamide's effect on the melting temperature was found to be % GC-dependent, while purine stretches were also found to influence the binding kinetics

SUBMITTER: Rasis M 

PROVIDER: S-EPMC12298274 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature