Immunogenicity of DNA, mRNA and Subunit Vaccines Against Beak and Feather Disease Virus.

ABSTRACT:

Background/objectives

Beak and feather disease virus (BFDV) is the causative agent of psittacine beak and feather disease (PBFD), affecting psittacine birds. There is currently no commercial vaccine or treatment for this disease. This study developed a novel BFDV coat protein mRNA vaccine encapsidated by TMV coat protein to form pseudovirions (PsVs) and tested its immunogenicity alongside BFDV coat protein (CP) subunit and DNA vaccine candidates.

Methods

mRNA and BFDV CP subunit vaccine candidates were produced in Nicotiana benthamiana and subsequently purified using PEG precipitation and gradient ultracentrifugation, respectively. The DNA vaccine candidate was produced in E. coli cells harbouring a plasmid with a BFDV1.1mer pseudogenome. Immunogenicity of the

SUBMITTER: Ndlovu B 

PROVIDER: S-EPMC12298428 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature