Snapshot Quantitative Phase Imaging with Acousto-Optic Chromatic Aberration Control.

ABSTRACT: The transport of intensity equation enables quantitative phase imaging from only two axially displaced intensity images, facilitating the characterization of low-contrast samples like cells and microorganisms. However, the rapid selection of the correct defocused planes, crucial for real-time phase imaging of dynamic events, remains challenging. Additionally, the different images are normally acquired sequentially, further limiting phase-reconstruction speed. Here, we report on a system that addresses these issues and enables user-tuned defocusing with snapshot phase retrieval. Our approach is based on combining multi-color pulsed illumination with acousto-optic defocusing for microsecond-scale chromatic aberration control. By illuminating each plane with a different color and using a colo

SUBMITTER: Alexandropoulos C 

PROVIDER: S-EPMC12298500 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature