Development of a Strain-Specific Detection and Quantification Method for <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> HN019 Using WGS-SNP Analysis and qPCR.

ABSTRACT: Accurate quantification of Bifidobacterium animalis subsp. lactis HN019, a clinically validated probiotic strain conferring immune modulation, gastrointestinal health, and gut barrier integrity benefits, is essential for diverse applications. To address the critical need for strain-specific detection, we developed a quantitative PCR (qPCR) assay targeting a unique single-nucleotide polymorphism (SNP) within the galK gene, identified through comparative whole-genome sequencing (WGS) analysis of 31 B. animalis subsp. lactis strains. The assay exhibited exceptional specificity, distinguishing HN019 from 19 other Bifidobacterium strains. Sensitivity tests indicated a detection limit of 0.5 pg of DNA and 10³ CFU/mL of bacterial cells, making

SUBMITTER: Mao D 

PROVIDER: S-EPMC12298522 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature