Comprehensive Analysis of Mycotoxins in Green Coffee Food Supplements: Method Development, Occurrence, and Health Risk Assessment.

ABSTRACT: This study investigates the presence of mycotoxins in green coffee-based dietary supplements to ensure their safety, given the potential risks of contamination and the growing interest in them among consumers. A sample treatment based on a salting-out assisted liquid-liquid extraction (SALLE) followed by one-step solid-phase extraction (SPE) was selected for the extraction and clean-up of 15 mycotoxins followed by ultra-high performance chromatography-tandem mass spectrometry detection (UHPLC-MS/MS). The target mycotoxins included aflatoxins (AFG1, AFG2, AFB1, AFB2), Alternaria toxins (AOH, AME, TEN), ochratoxin A (OTA), fumonisins (FB1, FB2), zearalenone (ZEN), trichothecenes (T-2, HT-2), enniatin B1 (ENNB1), and beauvericin (BEA). The proposed method was successfully characterized

SUBMITTER: Carbonell-Rozas L 

PROVIDER: S-EPMC12298769 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature