Functional Analysis of Zinc Finger Protein Transcription Factor <i>ZmZFP69</i> Under Low-Temperature Stress at Maize Seedling Stage.

ABSTRACT: Maize (Zea mays L.) seedlings are highly susceptible to low-temperature stress, which significantly impacts maize yield and quality. A zinc finger protein transcription factor (ZmZFP69) mutant and a control (B73) maize inbred line were subjected to low-temperature treatment, and changes in the phenotypic characteristics, hormone levels, and other indicators before and after the treatment were systematically identified. Subsequently, a combined RNA-seq and DAP-seq analysis was conducted to explore the influence of ZmZFP69 on the promoters of downstream genes. Finally, the proteins interacting with ZmZFP69 were examined using InterProDesign combined with BiFC and subcellular localization. The zmzfp69 homozygous mutant maize inbred line exhibited enhanced low-temperature

SUBMITTER: Li SN 

PROVIDER: S-EPMC12298789 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature